Great Fungal Barcoding Primers


Primer name Sequence Lab/commenter Tax group


ITS

  1. ITS1 5'-TCCGTAGGTGAACCTGCGG-3' forward all all
  2. ITS 1F 5'-CTTGGTCATTTAGAGGAAGTAA-3' forward all all
  3. ITS4 5'-TCCTCCGCTTATTGATATGC-3' reverse all all
  4. ITS4BR 5'-TCAACAGACTTGTACATGGTCC-3' reverse Hamelin
  5. ITS4BR2 5'-GGATTATCACCCTCAATGAT-3' reverse Hamelin
  6. ITS3R2 5'-AAGGTACACCTGTTTGAGTGTC-3' forward Hamelin
  7. ITS2R2 5'-GACACTCAAACAGGTGTACCTT-3' reverse Hamelin
  8. ITS5 5'-GGAAGTAAAAGTCGTAACAAGG-3' forward Seifert
  9. ITS3R3 5'-CACATCGATGAAGAACACAGT-3' forward Hamelin
  10. 5.8S 5'-CGCTGCGTTCTTCATCG-3' forward Moncalvo
  11. 5.8SR 5'-TCGATGAAGAACGCAGCG-3' reverse Moncalvo
  12. UN-UP18S42 5'-CGTAACAAGGTTTCCGTAGGTGAAC-3' forward Levesque
  13. UN-LO28S22` 5'-GTTTCTTTTCCTCCGCTTATTGATATG-3' reverse Levesque


LSU

  1. LR0R 5'-ACCCGCTGAACTTAAGC-3' forward
  2. LR5 5'-TCCTGAGGGAAACTTCG-3' reverse
  3. LR16 5'-TTCCACCCAAACACTCG-3' reverse


SSU

  1. SR1R 5'-TACCTGGTTGATQCTGCCAGT-3' forward James Chytrids/Zygos, all
  2. NS1 5'-GTAGTCATATGCTTGTCTC-3' forward all Ascomycota, all
  3. NS4 5'-CTTCCGTCAATTCCTTTAAG-3' reverse all Ascomycota, all
  4. PNS1 5’-CCAAGCTTGAATTCGTAGTCATATGCTTGTC-3’ forward Hibbett Basidiomycota
  5. NS41 5’-CCCGTGTTGAGTCAAATTA-3’ reverse Hibbett Basidiomycota


RPB1

  1. RPB1-Af, RPB1-Ac 5’-GARTGYCCDGGDCAYTTYGG-3’ forward Sung, Matheny all
  2. RPB1-Cr 5’-CCNGCDATNTCRTTRTCCATRTA-3’ reverse Sung, Matheny all


RPB2

  1. fRPB2-5F GAYGAYMGWGATCAYTTYGG forward Matheny all
  2. RPB2-7R CCCATWGCYTGCTTMCCCAT reverse Sung all
  3. bRPB2-7.1R 5'-CCCATRGCYTGYTTMCCCATDGC-3' reverse Matheny Basidiomycota


MCM7

  1. Mcm7-709for 5’-ACIMGIGTITCVGAYGTHAARCC-3’ forward Schmitt all
  2. Mcm7-1348rev 5’-GAYTTDGCIACICCIGGRTCWCCCAT-3’ reverse Schmitt all
  3. Mcm7-1447rev 5’-CATIACIGCIGCIGTRAGRCC-3’ reverse Schmitt all