Fungal Barcoding Primers

April 11, 2011

primer name sequence Lab/commenter Tax group
ITS
ITS1  5'-TCCGTAGGTGAACCTGCGG-3' forward all all
ITS 1F 5'-CTTGGTCATTTAGAGGAAGTAA-3' forward all all
ITS4 5'-TCCTCCGCTTATTGATATGC-3' reverse all all
ITS4BR 5'-TCAACAGACTTGTACATGGTCC-3' reverse Hamelin
ITS4BR2 5'-GGATTATCACCCTCAATGAT-3' reverse Hamelin
ITS3R2 5'-AAGGTACACCTGTTTGAGTGTC-3' forward Hamelin
ITS2R2 5'-GACACTCAAACAGGTGTACCTT-3' reverse Hamelin
ITS5 5'-GGAAGTAAAAGTCGTAACAAGG-3' forward Seifert
ITS3R3 5'-CACATCGATGAAGAACACAGT-3' forward Hamelin
5.8S 5'-CGCTGCGTTCTTCATCG-3' forward Moncalvo
5.8SR 5'-TCGATGAAGAACGCAGCG-3' reverse Moncalvo
UN-UP18S42 5'-CGTAACAAGGTTTCCGTAGGTGAAC-3' forward Levesque
UN-LO28S22` 5'-GTTTCTTTTCCTCCGCTTATTGATATG-3' reverse Levesque
LSU
LR0R 5'-ACCCGCTGAACTTAAGC-3' forward
LR5 5'-TCCTGAGGGAAACTTCG-3' reverse
LR16 5'-TTCCACCCAAACACTCG-3' reverse
SSU
SR1R 5'-TACCTGGTTGATQCTGCCAGT-3' forward James Chytrids/Zygos, all
NS1 5'-GTAGTCATATGCTTGTCTC-3' forward all Ascomycota, all
NS4 5'-CTTCCGTCAATTCCTTTAAG-3' reverse all Ascomycota, all
PNS1 5’-CCAAGCTTGAATTCGTAGTCATATGCTTGTC-3’ forward Hibbett Basidiomycota
NS41 5’-CCCGTGTTGAGTCAAATTA-3’ reverse Hibbett Basidiomycota
RPB1
RPB1-Af, RPB1-Ac 5’-GARTGYCCDGGDCAYTTYGG-3’ forward Sung, Matheny all
RPB1-Cr 5’-CCNGCDATNTCRTTRTCCATRTA-3’ reverse Sung, Matheny all
RPB2
fRPB2-5F GAYGAYMGWGATCAYTTYGG forward Matheny all
RPB2-7R CCCATWGCYTGCTTMCCCAT reverse Sung all
bRPB2-7.1R 5'-CCCATRGCYTGYTTMCCCATDGC-3' reverse Matheny Basidiomycota
MCM7
Mcm7-709for 5’-ACIMGIGTITCVGAYGTHAARCC-3’ forward Schmitt all
Mcm7-1348rev 5’-GAYTTDGCIACICCIGGRTCWCCCAT-3’ reverse Schmitt all
Mcm7-1447rev 5’-CATIACIGCIGCIGTRAGRCC-3’ reverse Schmitt all